Recent Citations for FLICA Assay Kits

Our FLICA Assay Kits continue to be cited in various journals. Here is a selection of citations from last month.

Bakr A, Oing C, Köcher S, Borgmann K, Dornreiter I, Peterson C, Dikomey E, Mansour WY. March 2015. "Involvement of ATM in homologous recombination after end resection and RAD51 nucleofilament formation." Nucl. Acids Res. doi: 10.1093/nar/gkv160. [Full Text]

Colin DJ, Hain KO, Allan LA, Clarke PR. March 2015. "Cellular responses to a prolonged delay in mitosis are determined by a DNA damage response controlled by Bcl-2 family proteins." Open Biol. doi: 10.1098/rsob.140156. [Full Text]

Larena M, Holmgren J, Lebens M, Terrinoni M, Lundgren A. March 2015. "Cholera Toxin, and the Related Nontoxic Adjuvants mmCT and dmLT, Promote Human Th17 Responses via Cyclic AMP-Protein Kinase A and Inflammasome-Dependent IL-1 Signaling." J. Immunol. doi:10.4049/jimmunol.1401633. [Abstract]

Products Used:

• FAM FLICA Poly Caspase Assay Kit (ICT# 91, 92)
• FAM FLICA Caspase 3 & 7 Assay Kit (ICT# 93, 94)
• FAM FLICA Caspase-1 Assay Kit (ICT# 97, 98)

Recent Customer Citations Using Magic Red Assay Kits

Our Magic Red products were recently cited in several publications.

Liu Y, Palanivel R, Rai E, Park M, Gabor TV, Scheid MP, Xu A, Sweeney G. January 2015. Adiponectin Stimulates Autophagy and Reduces Oxidative Stress to Enhance Insulin Sensitivity During High-Fat Diet Feeding in Mice. Diabetes. doi:10.2337/dlb14-0267. [Abstract]

Deffit SN, Blum JS. February 2015. Macronutrient Deprivation Modulates Antigen Trafficking and Immune Recognition through HSC70 Accessibility. J. Immunol. doi:10.4049/jimmunol.1402472. [Abstract]

Weikel KA, Cacicedo JM, Ruderman NB, Ido Y. February 2015. Glucose and Palmitate uncouple AMPK from autophagy in human aortic endothelial cells. Am. J. Cell. Physiol-Cell CP. doi:10.1152/ajpcell.00265.2014 [Full Text]

Products Used:

• Magic Red Cathepsin B Assay Kit (ICT# 937, 938)
• Magic Red Cathepsin L Assay Kit (ICT# 941, 942)

January 2015 Citations for FLICA Assay Kits

Two new citations using our FLICA Assay kits were published this month.
Identification of Aim2 as a Sensor for DNA Vaccines. J. Immunol. vol 194 no. 2 630-636. [Abstract]

"Samples were then stained with a caspase-1 FAM/FLICA kit according to the manufacturers [sic] instructions (ImmunoChemistry Technologies, Bloomington, MN)."

 Paster, W, Bruger, AM, Katsch, K, Gregoire, C, Roncagalli, R, Fu, G, Gascoigne, NRJ, Nika, K, Cohnen, A, Feller, SM, Simister, PC, Molder, KC, Cordoba, SP, Dushek, O, Malissen, B, Acuto, O. (2015). A THEMIS:SHP1 complex promotes T-cell survival. EMBO Journal 10.15252/embj.201387725[Full Text]

"A FAM‐FLICA detection probe was used to assess poly‐caspase activity by flow cytometry."

Products Used:

• FAM-FLICA Caspase 1 Assay Kit (ICT#97, 98)
• FAM-FLICA Poly-Caspase Assay Kit (ICT#91, 92)

Recent Customer Citations for Magic Red Cathepsin B Assay Kits

Li, K, Yang, L, Zhang, C, Niu, Y, Wei, L, Liu, J. (2014). HPS6 interacts with dynactin p150Glued to mediate retrograde trafficking and maturation of lysosomes. J. Cell Sci. 127, 4574-4588. [Abstract]

"we incubated cells with Magic Red cathepsin B (MR catB), a membrane permeable probe that fluoresces..."

Bain, JM, Louw, J, Lewis, LE, Okai, B, Walls, CA, Ballou, ER, Walker, LA, Reid, D, Munro, CA, Brown, AJP, Brown, GD, Gow, NAR, Erwig, LP. (2014). Candida albicans Hypha Formation and Mannan Masking of β-Glucan Inhibit Macrophage Phagosome Maturation. mBio. vol. 5 no. 6 e01874-14. [Full Text]

Abstract:

Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host. Host defense against systemic Candida infection relies heavily on the capacity of professional phagocytes of the innate immune system to ingest and destroy fungal cells. A number of pathogens, including C. albicans, have evolved mechanisms that attenuate the efficiency of phagosome-mediated inactivation, promoting their survival and replication within the host. Here we visualize host-pathogen interactions using live-cell imaging and show that viable, but not heat- or UV-killed C. albicans cells profoundly delay phagosome maturation in macrophage cell lines and primary macrophages. The ability of C. albicans to delay phagosome maturation is dependent on cell wall composition and fungal morphology. Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes. The loss of cell wall O-mannan leads to exposure of β-glucan in the inner cell wall, facilitating recognition by Dectin-1, which is associated with enhanced phagosome maturation.

Products Used:

• Magic Red Cathepsin B Assay Kit (ICT# 937, 938)

September Publications Citing FLICA® 660 Caspase Assays for Apoptosis Detection

Saunders, AE, Shim, YA, Johnson, P. (2014). Innate Immune Cell CD45 Regulates Lymphopenia-Induced T Cell Proliferation. J. Immunol. 193(6). 2831-2842. [Abstract]

"...The presence of active caspase-3 and -7 was assessed using FLICA 660 assay kit (Immunochemistry Technologies) in accordance with the manufacturers {sic} instructions..."

Samejima, K, Ogawa, H, Ageichik, AV, Peterson, KL, Kaufmann, SH, Kanemaki, MT, Earnshaw, WC. (2014). Auxin-induced Rapid Degradation of Inhibitor of Caspase-activated DNase (ICAD) Induces Apoptotic DNA Fragmentation, Caspase Activation, and Cell Death. J. Biol. Chem. 289, 31617-31623. [Abstract]

“…Caspase activation was analyzed using the FLICA 660 in vitro poly caspase detection kit (ImmunoChemistry Technologies LLC) following the manufacturer’s instructions…”

Products Cited:
FLICA 660 Caspase 3/7 Kit (ICT# 9125)
FLICA 660 Poly Caspase Assay Kit (ICT# 9120)

Recent Customer Citations Using FLICA® Caspase-1 Assay Kits

Recently, our customers have published two research articles using our FLICA® Kits to detect caspase-1 activity.

The first article, authored by researchers at the Utrecht University Department of Infectious Diseases and Immunology, studied the pathogen that causes foodborne disease.

Recent Customer Publication Using FLICA Assays

Researchers at Harvard Medical School and  Schepens Eye Research Institute recently published a study using several of our FLICA apoptosis assays to detect caspase activity in retinal pigment epithelial cells.

Kim LA., Amarnani D, Gnanaguru G, Tsen WA, Vavvas DG, and D'Amore PA. (2014.) Tamoxifen Toxicity in Cultured Retinal Pigment Epithelial Cells Is Mediated by Concurrent Regulated Cell Death Mechanisms. Invest. Ophthalmol. Vis. Sci., 55(8), 4747-4758. [Abstract]

"Caspase activity was evaluated by caspase-1, -3, -8, and -9 specific inhibition... Caspase activity was verified with the fluorochrome-labeled inhibitor of caspases (FLICA) probe specific for each caspase."